Device

Part:BBa_K317030:Design

Designed by: Yoshiyuki MURAKAMI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-20)
P(J23102)-RBS(B0034)-pduP_GFP-Term(B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 635
    Illegal XhoI site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used following 3 primers to construct the fusion protein of N-terminal 18 amino acids of pduP and GFPuv.


pduP-1  : 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAACACTT-3'

pduP-2 : 5'-GAGGAGAAATACTAGATGAACACTTCAGAACTTGAAACCCTTATTCGTAACATTTTGA-3'

pduP-3 : 5'-TGAAAAGTTCTTCTCCTTTACTCATAAGTTGCTCACTCAAAATGTTACGAATAAGGGTTTC-3'


References